We provide Next generation sequencing (NGS) with a variety of platforms (GS-FLX Titanium, SOLEXA GA IIx, SOLEXA HiSeq 2000, ABI SOLiD v.4).
With these cutting-edge platforms, we are able to do a number of NGS applications:

• De novo and Re-sequencing
  • We provide ultra-high throughput and broad range of whole genome sequencing including de novo assembly and re-sequencing using powerful combination of read-length and paired-end flexibility. We accept a variety of organisms including human, monkey, rat, mouse, microorganism (fungus, yeast, bacteria), plant etc.
• Targeted Sequencing
  • Targeted sequencing is a sequencing method that captures specific regions of interest and sequence only those regions. We provide a various services of targeted sequencing including chromatin immunoprecipitation sequencing(ChIP-seq), custom capture sequencing and exome sequencing.
• cDNA / Transcriptome Analysis
  • Transcriptome sequencing using NGS is a fast and reliable method to identify genomic information in known and novel organisms. We provide whole mRNA transcript expression analysis (full-length mRNA and EST), novel gene discovery, identification of SNP.
• Methylation Sequencing
  • Methylation in the chromosomal DNA is one of the methods to regulate gene expression. To characterize methylated base is to understand one of gene regulation. In methylation experiments, applying a standard bisulfite-treatment procedure, we identify the quantitative characterization of the methylation state of each CpG dinucleotide in a given target genomic sequence. For this approach DNA is treated with sodium bisulfite, resulting in the deamination of unmethylated cytosines to uracils, while methylated cytosines remain unchanged. Subsequent PCR amplification of the converted cytosine (now uracil) results in the substitution of thymine for the uracil. Comparison of the sequence obtained from the bisulfite-treated amplicon to the published sequence enabling identification of any differential methylation. Using our NGS systems, we provide methylation sites in the chromosomal DNA.
• Small RNA Discovery and Analysis
  • Small RNA sequencing using NGS systems is a powerful application to identify and profile miRNA, siRNA, piRNA and other non-coding RNA on any organism. We provide rapid sequencing of small RNAs for rapid identification and quantification without cloning into bacteria.
• Metagenomics and Diversity Analysis
  • Metagenome means all the genetic material present in an environmental sample including soil samples, several microenvironments within the human body and the various layers within the ocean. The purposes of metagenome sequencing are to identify the organisms present in a sample and characterize the roles in the specific environment.

Recommended Platforms and Applications:
Please send in and inquiry to us for more information.